Depth Chart
| Offense | |||
| Position | |||
| Left Tackle | Trent Williams | Jaylon Moore | Sam Schlueter |
| Left Guard | Colton McKivitz | Spencer Burford | Justin Skule |
| Center | Dohnovan West | Jake Brendel | |
| Right Guard | Daniel Brunskill | Aaron Banks | Keaton Sutherland |
| Right Tackle | Mike McGlinchey | Nick Zakelj | Alfredo Gutierrez |
| Wide Receiver | Deebo Samuel | Jauan Jennings | KeeSean Johnson |
| Wide Receiver | Brandon Aiyuk | Danny Gray | Marcus Johnson |
| Running Back | Eli Mitchell | Tyrion Davis-Price | Jeff Wilson Jr. |
| Tight End | George Kittle | Ross Dwelley | Charlie Woerner |
| Quarterback | Jimmy Garoppolo | Trey Lance | Nate Sudfeld |
| Defense | |||
| Position | |||
| Left Defensive End | Dee Ford | Samson Ebukam | Kemoko Turay |
| Left Defensive Tackle | Javon Kinlaw | Kevin Givens | Maurice Hurst |
| Right Defensive Tackle | Arik Armstead | Hassan Ridgeway | Kalia Davis |
| Right Defensive End | Nick Bosa | Drake Jackson | Kerry Hyder Jr. |
| Strong Linebacker | Azeez Al-Shaair | Segun Olubi | Marcelino McCrary-Ball |
| Middle Linebacker | Fred Warner | Oren Burks | Jeremiah Gemmel |
| Weak Linebacker | Dre Greenlaw | Demetrius Flannigan-Fowles | Curtis Robinson |
| Left Cornerback | Ambry Thomas | Emmanuel Moseley | Jason Verrett |
| Right Cornerback | Charvarius Ward | Dontae Johnson | Darqueze Dennard |
| Strong Safety | Talanoa Hufanga | George Odum | Leon O'Neal Jr. |
| Free Safety | Jimmie Ward | Tarvarius Moore | Deommodore Lenoir |
| Special Teams | |||
| Position | |||
| Kicker | Robbie Gould | ||
| Kick-Offs | Robbie Gould | ||
| Punter | Mitch Wishnowsky | ||
| Kick Returner | Ray-Ray McCloud III | Ambry Thomas | JaMycal Hasty |
| Punt Returner | Ray-Ray McCloud III | Brandon Aiyuk | JaMycal Hasty |
| Holder | Mitch Wishnowsky | ||
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