Depth Chart
| Offense | |||
| Position | |||
| Left Tackle | Trent Williams | Jaylon Moore | |
| Left Guard | Laken Tomlinson | Tom Compton | Aaron Banks |
| Center | Alex Mack | Jake Brendel | |
| Right Guard | Daniel Brunskill | ||
| Right Tackle | Mike McGlinchey | ||
| Wide Receiver | Brandon Aiyuk | Mohamed Sanu | Jauan Jennings |
| Wide Receiver | Deebo Samuel | Trent Sherfield | |
| Running Back | Eli Mitchell | Trey Sermon | Trenton Cannon |
| Tight End | George Kittle | Charlie Woerner | Ross Dwelley |
| Quarterback | Jimmy Garoppolo | Trey Lance | |
| Defense | |||
| Position | |||
| Left Defensive End | Nick Bosa | Kentavius Street | Arden Key |
| Left Defensive Tackle | Javon Kinlaw | D.J. Jones | |
| Right Defensive Tackle | Zach Kerr | ||
| Right Defensive End | Arik Armstead | Dee Ford | |
| Strong Linebacker | Samson Ebukam | ||
| Middle Linebacker | Fred Warner | Azeez Al-Shaair | |
| Weak Linebacker | Demetrius Flannigan-Fowles | ||
| Left Cornerback | K'Waun Williams | Deommodore Lenoir | Dre Kirkpatrick |
| Right Cornerback | Ambry Thomas | Josh Norman | Emmanuel Moseley |
| Strong Safety | Jaquiski Tartt | Marcell Harris | Tavon Wilson |
| Free Safety | Jimmie Ward | Talanoa Hufanga | |
| Special Teams | |||
| Position | |||
| Kicker | Robbie Gould | ||
| Kick-Offs | Robbie Gould | ||
| Punter | Mitch Wishnowsky | ||
| Kick Returner | Trent Sherfield | ||
| Punt Returner | Trent Sherfield | ||
| Holder | Mitch Wishnowsky | ||
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